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Ready-to-use diluents Dilucup

Let's explore the 3 most used Dilucup® diluents for making serial dilutions:

  • Maximum Recovery Diluent (MRD):

MRD is an isotonic diluent formulated for the optimal recovery of microorganisms. By combining the protective effects of peptic digest with the osmotic balance of physiological saline, MRD ensures the viability of organisms without allowing them to multiply for a short period. MRD is extensively used in various sectors, including food safety, environmental testing or veterinary applications, among others.

  • Buffered Peptone Water (BPW):

BPW serves as a non-selective pre-enrichment medium, widely used for recovering sub-lethally damaged microorganisms, particularly Salmonella spp., from food samples. Its buffered nature maintains a stable pH, essential for the recovery and growth of these microorganisms, ensuring that they are in optimal condition for further analysis.

  • Saline Solution (NACL):

Known commonly as salt, NaCl is used in laboratories for maintaining osmotic balance in solutions. Its stability and non-toxic nature make it ideal for supporting the growth and maintenance of microorganisms without causing osmotic stress.

Whether you’re involved in food safety, the probiotic or cosmetic industries, environmental testing, paper manufacturing or process water treatment, these Dilucup® diluents are designed to meet your needs.

Dilucup® Elegance
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ready to use dilution media

Have you thought about the time and resources spent on preparing dilution tubes?

The process involves calibrating instruments, mixing chemicals, autoclaving, performing quality control using bacterial strains and finally dispensing into tubes.

If you're lucky and have done everything perfectly, you can use the tubes directly. However, this is often not the case. Risks like cross-contamination or incorrect diluent volumes can lead to discarding entire batches and starting over, wasting valuable time and resources.

In addition, lab-prepared tubes require refrigeration which limits batch sizes. So, you'll likely have to repeat this process every week.

Now, imagine a more efficient solution: simply open a bag, grab the ready-to-use dilution cups, place them on the Dilugent® Shaker and start diluting.

Practical and reliable.

Let us handle the heavy lifting so you can focus on diluting your samples with peace of mind!

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accurate serials dilutions

Dilucup® Elegance is the ultimate solution for accurate and efficient serial dilutions.

Here's why it's a favourite in modern laboratories:

  • Ready-to-use: no preparation, calibration, refilling or cleaning required ⇒ Convenient + save time and resources.
  • Highly accurate diluent volumes: 9 mL ± 0.5% ⇒ Ensures precise results.
  • Long shelf life of 30 months after production ⇒ Keeps your lab stocked and ready.
  • Compact design ⇒ Saves half the space compared to dilution tubes.
  • ISO compliant: meets ISO standards and is mentioned as an alternative in the latest revision ISO 6887-1:2017/Amd 1:2024. ⇒ Standardized dilutions.
  • User-friendly: the cups are easily opened with just two fingers thanks to a thin, easily removable protective film. ⇒ Comfortable to use.
  • High-speed vortexing: the cups can be shaken at high speed (660 rpm) without spilling. ⇒ Ensures homogeneity of the samples and therefore reliable results.
  • Diluents available: MRD, NACL, BPW, EP and DW, with custom options available upon request. ⇒ Versatile, useful in many industries.
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serial dilutions Dilucup vs dilution tubes

Watch the video and compare the Dilucup® System vs dilution tubes and a vortex mixer.

In addition to saving time, the Dilucup® System offers you much more.

⬅️ The user on the left simply had to place the ready-to-use dilution cups on the shaker and start diluting. She didn't have to prepare, perform QC, calibrate or clean anything. Plus, she always makes the dilutions exactly the same way, so they're standardized.

Now look at the user on the right.
➡️The process is much more laborious. Every step requires her full attention: opening and closing the caps, tilting the tube correctly to achieve an internal vortex that ensures proper homogenization and counting the seconds of steering all the time. This means that dilutions may vary greatly, especially among different users, so they are not standardized.

Benefits
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how to make 7 dilutions

Flexibility is at the core of Dilugent® Shaker. Need to adjust the number of dilutions?

No problem!

You can handle from 1 to 12 dilutions per sample with ease. Simply divide the Dilucups blister to fit the number of dilutions you need, and that's it!

But wait, there's more!

Dilugent® Shaker isn’t just about flexibility; it’s about comfort too. Enjoy the ease of pipetting with a design that feels just right and activate the shaking with a convenient foot pedal, as shown in the video.

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To access the manual, please log in with your user account and download it the following links:

Do you also want the manual in your language?

Contact us and we will be happy to translate it for you.

Contact us
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highly accurate diluent volumes Dilucup

Every time we review the statistics for the volume of diluent in Dilucup®, the results continue to improve. Our latest evaluation in February covered the last controlled dilution cups (more than 15,000), and the results speak for themselves:

✔ 99.1% of Dilucups contained 9 mL ± 0.5%

✔ 99.9% had a volume of 9 ± 1%

According to ISO 6887-1:2017/Amd 1:2024 the volume of diluent cannot exceed a deviation of ± 2%.

🔬 With the Dilucup® System you dilute your samples with the certainty of obtaining reliable enumerations.

Dilucup® Elegance
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colourblind dilutions series

Last week, when I visited a customer at their lab, I met a man who refused to perform serial dilutions in any other way than with tubes.
He was used to them and although it took a lot of time and effort to prepare them, he had worked like that for 10 years and had no intention of changing.

It turns out that when I showed him the Dilucup® System he got impressed:

-“It's clear that I could save a lot of time with Dilucup®” - he said. And he continued – “But I don't understand the colour code, what is it for?”

I explained that the red Dilucups were the ones that had been diluted and plated in the previous sample. The orange ones are those ready to be plated from the actual sample and the green ones those to be used in the next sample.

-“But I only see 2 different colours and I will get confused. I am colour-blind, you know, and this does not work for me.”

-“No problem” - I said.
Then I changed the colours.

He chuckled.

-“Wow, this changes everything. Now I can see the 3 colours!” - he said.

Read more about Dilugent Shaker
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Serielle verdünnungen Verdünnungsreihen diluizioni-seriali diluições em série seriefortynninger sarjalaimennokset rozcieńczenia seryjne raðþynningum seriële verdunningen Serijska razrjeđenja σειριακές αραιώσεις serielle-verdunnungen Serijska redčenja серијска разблажења sériová ředění Sériové riedenia серийни разреждания Cеријски разредувања

Click on the different links to read in your language how the Dilucup® System can help you improve serial dilutions.

Bulgarian
Cерийни разреждания

 Croatian
Serijska razrjeđenja

 Czech
Sériová ředění

 Dutch
Seriële verdunningen

 Finnish
Sarjalaimennokset

 German
Serielle verdünnungen
Verdünnungsreihen

 Greek
σειριακές αραιώσεις

 Hungarian
Sorozatos hígítások

 Icelandic
Raðþynningum

Italian
Diluizioni-seriali

 Japanese
連続希釈

 Korean
연속 희석

 Luxembourgish 
Serielle-verdunnungen

 Macedonian
Cеријски разредувања

 Norwegian
Seriefortynninger

 Polish
Rozcieńczenia seryjne

Portuguese
Diluições em série

 Romanian
Diluții în serie

 Serbian
Cеријска разблажења

 Slovak
Sériové riedenia

 Slovenian
Serijska redčenja

 Ukrainian
Cерійні розведення

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total count method

How to enumerate microorganisms in food products?

Total count method:

  1. Prepare the sample:
  • If it is solid: take a small quantity and homogenize it with a diluent so that you have a first dilution 1/10.
    For instance: 10 g of minced meat + 90 mL of MRD (Maximum Recovery Diluent).
  • If your sample is already liquid, skip this step.
  1. Perform serial dilutions with the Dilucup® System to reduce the concentration of microorganisms to a countable range.
  2. Plate and incubate: place 1 mL of the diluted suspensions on agar plates or equivalent and incubate them. This step will allow microorganisms to become visible colonies.
  3. Count the colonies after incubation. Plates should have between 30 and 300 Colony-Forming Units (CFU) to ensure statistical validity.
  4. Calculate the number of microorganisms per millilitre or gram in the original sample by dividing the number of CFU by the dilution factor.
    For example: 131 CFU / 10-2 = 1,31 x 104 CFU/g.

Enumerating microorganisms in food products is an essential analysis to ensure the quality of the product and its safety for consumption.

With the Dilucup® System you save a lot of time with serial dilutions and get accurate results.