Ensure homogeneous mixing
The interior of the Dilucup® is shaken thoroughly with one the two available laboratory shakers, Dilushaker III or Dilugent Shaker. Thanks to the clever design of the cup, it can rotate at high speeds (400 - 660 rpm) without spilling, thus ensuring homogeneous mixing.
To begin, simply place the blister with Dilucups on top of the shaker. Next, open the number of cups you intend to use by tearing off a thin film. This layer on the top not only guarantees sterility but also ensures the correct volume inside the cups.
You can use a cutter to cut the film or scissors to cut the whole blister to separate the cups.
Trace your dilutions
Tracing the methods in the lab is of utmost importance in order to be able to look back when there is a mistake and investigate why it happened.
To facilitate this, both the blisters and the bags of Dilucup® are equipped with QR codes that can be easily scanned by Dilugent® Shaker. Furthermore, you can extract the information with a USB stick and subsequently store it on the computer.
How many samples?
Dilucup® Elegance is available in blisters of seven rows of either three (7x3) or six cups (7x6).
Depending on the number of dilutions per sample you usually perform, you may find the small or large blister more convenient.
For example, if you usually perform 3 dilutions, we recommend the blister with 7x3 Dilucups. However, if you usually perform more than 3 dilutions, then the larger blister with 7x6 cups will probably work best for you.
Both versions are designed to perfectly fit on the tray of Dilushaker III and Dilugent® Shaker.
Diluents
The following features apply to all our standard pre-filled dilution cups except DW:
Volume:
pH:
Microbiological productivity:
Quantity:
3 cups / row:
6 cups / row:
9 mL ± 2 % (according to ISO 6887-1:2017)
7,0 ± 0,2 (according to ISO 6887-1:2017)
verified with E. coli and S. aureus (according to ISO 11133:2014)
672 total cups/box
2 bags x 16 blisters x 21 Dilucups per box
2 bags x 8 blisters x 42 Dilucups per box
NACL
Saline solution /
Salt diluent
Buffered solution including
NaCl 8,5 g/L
40DENACL3: 3 cups/row
40DENACL6: 6 cups/row
MRD
Maximal Recovery Diluent / Peptone Salt
Buffered solution including peptones 1 g/L, NaCl 8,5 g/L
40DEMRD3: 3 cups/row
40DEMRD6: 6 cups/row
BPW
Buffered Peptone Water / Phosphate Water
Buffered solution including peptones 10 g/L, NaCl 5 g/L
40DEBPW3: 3 cups/row
40DEBPW6: 6 cups/row
EP
Eau peptonée /
Peptone Water
Buffered solution including
peptone 0,1%
40DEEP3: 3 cups/row
40DEEP6: 6 cups/row
DW
Deionized Water
deionized water
Composition: Deionized water
Conductivity: less than 10 μS/cm
40DEDW3: 3 cups/row
40DEDW6: 6 cups/row