Dilucup® Elegance.

Optimize your serial dilution process.

Dilucup® Elegance is a prefilled container with a sterile diluent used in serial dilution in combination with one of the purpose-built shakers, Dilushaker III or Dilugent®Shaker.

Dilucup® Elegance allows laboratories to standardize and secure the serial dilution process in for example Food Microbiology, Bio Industry and Paper Industry.

 

serial dilution dilucup
Easy as 1-2-3

1. Add 1 mL sample to the first Dilucup®
2. Dilushake it
3. Collect and release 1 mL from the first cup to the next

LabRobot Products AB has invented, developed and patented the Dilucup® System that makes serial dilutions easier, faster and more cost-effective.

 

Before the Dilucup® System, the common way to dilute microbiological samples 1:10, 1:100, 1:1000, etc. was to prepare own test tubes. These tubes were filled with a diluent, sterilized in an autoclave and then stored in a fridge. After use, the tubes needed to be emptied and washed in order to be ready to be filled again. This process was both time consuming and expensive. It could also happen that evaporation during autoclaving was uncontrolled, thus generating volume deviations able to affect the accuracy of the analysis.

The Dilucup® System allows standardization of the dilution process and reduces variability between technicians.

dilution standardization

A Dilucup® is a small container pre-filled with 9 or 9,9 mL of a sterile diluent. It is available in blisters of seven rows of either three or six containers, designed to fit in the tray of Dilushaker III or Dilugent® Shaker.

The design of the cups allows its rotation at high speed without spilling.

The user opens the requested number of Dilucups® by tearing-off a thin film that guarantees the sterility and the right volume in the cups.

dilucup features

Using the Dilucup® System you simply:

  1. Place the blister with Dilucups® on the shaker.
  2. Tear off the protective film.
  3. Add 1 mL sample to the first Dilucup®.
  4. Start the shaker by moving your hand in front of the optic sensor. The 1:10 dilution is now ready.
  5. With a new sterile pipette tip, collect 1 mL from the first cup and release it into the second cup in order to perform the next dilution 1:100.
  6. Start the shaker by moving your hand in front of the optic sensor.
  7. Repeat this operation until the final dilution rate is reached
Pick your diluent

Diluents

  • MRD (Maximum Recovery Diluent)
  • BPW (Buffered Peptone Water)
  • NaCl, 0.85%
How many samples?
Shaker
  • 6 rows for 42 cups (7 x 6)
  • 3 rows for 21 cups (7 x 3)
Accessories

Accessories

  • Removable tray
  • Footswitch
  • Transport suitcase

Save money

The reasonable cost of the ready-to-use Dilucups® combined with the significant increase of the laboratory efficiency is contributing positively to the lab economy.

Save time

The Dilucup® System reduces half the time compared with standard dilution tubes.

Prevent work injuries

All the shaking vibrations are absorbed by the shaker when using the Dilucup® System, this prevents possible injuries as Repetitive Strain Injuries with the repeated use of a vortex mixer.

Increase quality

This is obtained through the standardization of the dilution process (less variability) and the accurate volume of the diluent in the Dilucups®.

Easy storage

The sealed bags with Dilucups® can be stored at room temperature up to 30 months.
Bags that are open can be stored for up to 3 months refrigerated in the fridge.

Diluents.

DILUCUP ELEGANCE

LabRobot offers a range of 3 standard Dilucup® Elegance products, but other diluents can be ordered on request.

Dilucup® Elegance MRD
(Maximal Recovery Diluent)

Composition: Peptones 1 g/L, NaCl 8,5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
40DEMRD3: 3 cups/row (2 bags x 16 blisters/bag x 21 cups/blisters)
40DEMRD6: 6 cups/row (2 bags x 8 blisters/bag x 42 cups/blisters)

Dilucup® Elegance BPW
(Buffered Peptone Water)

Composition: Peptones 10 g/L, NaCl 5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
40DEBPW3: 3 cups/row (2 bags x 16 blisters/bag x 21 cups/blisters)
40DEBPW6: 6 cups/row (2 bags x 8 blisters/bag x 42 cups/blisters)

Dilucup® Elegance NACL

Composition: NaCl 8,5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
40DENACL3: 3 cups/row (2 bags x 16 blisters/bag x 21 cups/blisters)
40DENACL6: 6 cups/row (2 bags x 8 blisters/bag x 42 cups/blisters)

The production of other articles (proprietary recipe, different volume, etc…) is possible with a minimum order of one pallet (21 504 cups). If interested, please contact us for a feasibility study.

How to enumerate the microorganisms present in a sample with Dilucup®?

  1. If the sample is solid, a first homogenisation step is required to prepare a 1/10 dilution.
  2. Once the sample is suspended or if the sample is liquid, serial dilute it with Dilucup®
  3. Plate 1 mL of the selected dilution(s) and incubate them.
  4. Count the colony-forming unit (CFUs). Remember that the plates should have between 30 and 300 CFUs to get a statistically valid result.
  5. Divide the average number of CFUs per the dilution level to get the number of microorganisms per mL or g in the original sample.
Shakers used with Dilucup® Elegance.