Serial Dilution System.
Optimize your serial dilution process.
Dilucup is a prefilled container with a sterile diluent used in combination with a purpose-built shaker, the Dilushaker.
Dilucup allows laboratories to standardize and secure the serial dilution process in for example Food Microbiology, Bio Industry and Paper Industry.
Easy as 1-2-3
1. Add 1 mL sample to the first Dilucup
2. Dilushake it
3. Collect and release 1 mL from the first cup to the next
LabRobot Products AB has invented, developed and patented the Dilucup System that makes serial dilutions easier, faster and more cost-effective.
The Dilucup System allows standardisation of the dilution process and reduces the
variability between technicians, often observed in traditional microbiology.
Some years ago, the common way to dilute microbiological samples 1:10, 1:100, 1:1000, etc. was to prepare own test tubes. These tubes were filled with a diluent (for example Pepton-Salt), sterilized in an autoclave and then stored in a fridge. After use, the tubes needed to be emptied and washed in order to be ready to be filled again. This process was both time consuming and expensive. It could also happen that evaporation during autoclaving was uncontrolled, thus generating volume deviations able to affect the accuracy of the analysis.
Using the Dilucup System you simply:
- Place the requested number of prefilled Dilucup on the Dilushaker
- Tear off the protective film
- Add 1 mL sample to the first Dilucup
- Start the shaker by moving your hand in front of the optic sensor
- When the Dilushaker stops, the 1:10 dilution is ready
- With a new pipette tip, collect 1 mL from the first cup and release it into the second cup in order to perform the next dilution 1:100
- Start the shaker by moving your hand in front of the optic sensor
- Repeat this operation until the final dilution rate is reached
Diluents
- MRD (Maximum Recovery Diluent)
- BPW (Buffered Peptone Water)
- NaCl, 0.85%
Shaker
- 6 rows for 42 cups (7 x 6)
- 3 rows for 21 cups (7 x 3)
Accessories
- Removable tray
- Footswitch
- Transport suitcase
Cost saving
The reasonable cost of the ready-to-use Dilucups combined with the significant increase of the laboratory efficiency is contributing positively to the lab economy.
Save time
It’s much quicker to perform dilutions with Dilucup than with standard dilution tubes. Time saving can be up to 10 seconds/dilution step.
Prevent work injuries
The repeated use of a vortex mixer can generate Repetitive Strain Injuries. With Dilucup, all the vibrations are absorbed by the Dilushaker. The use of plastic materials is also a safety factor, should something go wrong.
Increase quality
This is obtained through the standardization of the dilution process (less variability) and the accurate volume of the diluent in the Dilucups. All Dilucup batches are quality controlled and delivered with a QC Certificate.
Easy storage
The sealed Dilucup boxes can be stored at room temperature up to 20 months.
Diluents.
A Dilucup is a small container pre-filled with 9 mL of a sterile diluent. It is available in blisters of seven rows of either three or six containers, designed to fit in the tray of a dedicated “Dilushaker”. The user opens the requested number of Dilucups by tearing-off a thin film that guarantees the sterility and the right volume in the cups.
LabRobot offers a range of 3 standard Dilucup Elegance products.
Dilucup Elegance MRD
(Maximal Recovery Diluent)
Composition: Peptones 1 g/L, NaCl 8,5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
3 cups/row (2 x 16 x 21 cups)
40DEMRD3
6 cups/row (2 x 8 x 42 cups)
40DEMRD6
Dilucup Elegance BPW
(Buffered Peptone Water)
Composition: Peptones 10 g/L, NaCl 5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
3 cups/row (2 x 16 x 21 cups)
40DEBPW3
6 cups/row (2 x 8 x 42 cups)
40DEBPW6
Dilucup Elegance NACL
Composition: NaCl 8,5 g/L, Buffers
pH: 7,0 ± 0,2
Volume: 9 mL ± 2 %
Article numbers:
3 cups/row (2 x 16 x 21 cups)
40DENACL3
6 cups/row (2 x 8 x 42 cups)
40DENACL6
The production of other articles (proprietary recipe, different volume, etc…) is possible with a minimum order of one pallet (21 504 cups).
If interested, please contact us for a feasibility study.
Dilushaker.
The Dilushaker III Digital has been developed in order to guaranty a smooth and effective use of Dilucups.
The shaking is started immediately through the activation of an optic sensor located in the front of the device. Rotation speed and shaking time are pre-set by the user, so that every dilution is processed according to the same protocol (time + speed).
Specifications:
- Adjustable shaking time from 1 to 15 seconds per dilution step
- Adjustable rotation speed (400, 500 or 600 rpm)
- Removable tray for easy cleaning
- Ramp with LED lights to guide the user
- Adjustable direction of the ramp to suit both right- and left-handers
- Adjustable number of dilution steps per sample
- Steering by foot switch or optical sensor
- Ramp with 3 LED lights,
- Removable tray for 21 cups
- Recommended to be used with blisters of 3×7 Dilucups
Article number: 40DS21PRXT
- Ramp with 6 LED lights,
- Removable tray for 42 cups
- Recommended to be used with blisters of 6×7 Dilucups
Article number: 40DS42PRXT
The Dilushaker III Digital was exclusively developed for LabRobot. It is assembled and checked in our facilities in Stenungsund, Sweden.
Dilugent® Shaker.
Dilugent® Shaker is a new instrument for a safer and more traceable use of Dilucup® Elegance!
Dilugent® Shaker is designed to produce a more efficient and repeatable dilution workflow, in addition to saving up to 50% of the time spent with traditional methods.
The instrument is equipped with two sensors located on the sides that allow steering without physical contact. You choose which sensor is best for you, right or left, but you get also the option to plug a pedal and steer with a foot.
The 42 LEDs help during the dilution process by showing which cup to handle.
Dilugent® Shaker takes the use of Dilucup® Elegance to a higher level, with its 7” touchscreen ideal to easily program, control during use and export dilution data afterwards.
Specifications:
- Pipetting with Dilugent® Shaker is more convenient, because the tray is lower and the arm does not need to be as high.
- Easier adjustment of shaking time and rotation speed.
- Quicker shaking, up to 660 rpm, even with a 42 cup blister.
- Ability to change the number of dilutions between two samples with a simple click.
- Sensors placed on the sides prevent accidental shaking that can occur with Dilushaker III.
- Dilugent® Shaker makes it possible to control more parameters than Dilushaker III, e.g. sample, batch and user identification (not available in Light version).
- Dilugent® Shaker makes it possible to trace all dilutions as they are stored in an Excel sheet (not available in Light version).
- Dilugent Shaker allows safer use of Dilucup with 42 LEDs instead of 3 or 6 on Dilushaker III digital.
- With Dilugent® Shaker, it is possible to shake as many times as desired without affecting the LED lights (unthinkable with Dilushaker III digital).
- Dilushaker® Shaker is perfect for samples diluted in duplicate or in triplicate thanks to the "step forward" feature (only on the Pro version)
Article number:
Dilugent Shaker Light= 40DGT84LT
Dilugent Shaker (standard version) = 40DGT8
Dilugent Shaker Pro = 40DGT84PO
If you want to read more click on the link or contact us.