Elisabet Nuñez Gaitan

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how to enumerate microorganisms

How to enumerate the microorganisms present in a sample?

  1. If the sample is solid, a first homogenisation step is required to prepare a 1/10 dilution.
  2. Once the sample is suspended or if the sample is liquid, serial dilute it with Dilucup®
  3. Plate 1 mL of the selected suspension(s) and incubate them.
  4. Count the colony-forming unit (CFUs). Remember that the plates should have between 30 and 300 CFUs to get a statistically valid result.
  5. Divide the average number of CFUs per the dilution level to get the number of microorganisms per mL or g in the original sample.
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With Dilucup® Elegance don't worry about the volume of the diluent in the dilution process!

According to EN ISO 6887-1: 2017, the volume of the diluent cannot exceed a 2,0% deviation from 9,0mL. LabRobot still wants to be more accurate and that is why the volume in Dilucup® Elegance should never exceed 9,0ml ± 1,0%.

In 2020 none of the Dilucups manually controlled in our QC laboratory exceeded a ± 1% deviation from 9,0mL:

  • 90,9% had a deviation of 9,0 mL ± 0,5%
  • 0,3% between -0,5 and -1,0%
  • 8,8% between +0,5 and +1,0%

Do you know any other serial dilution method with greater volume precision?

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LabRobot was happy to participate in the Swedish movement “Håll Sverige Rent” (= Keep Sweden Tidy) and we dedicated a few minutes of our lunch break to collect plastic waste around our building.

If we all would do the same frequently, we would greatly reduce the amount of plastic materials in the nature and the oceans!

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That is how Dilucup Elegance can look like during the serial dilution process.

Did you know that you can customize the colours of the LED lights of a Dilugent Shaker Pro?

Dilugent® Shaker
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At LabRobot we don´t get sweets in Easter eggs, but Dilucups filled with exactly 9 mL of diluent!!

We wish you a happy and well-deserved Easter!

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Did you receive our newsletter?

Yesterday we sent a new edition that will surely interest you as a Dilucup user. Nobody likes spam, neither do we, that’s why we only send relevant information to our customers and distributors.

Not yet a subscriber? Subscribe immediately in the home page!

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We are proud to be part of AddLife, a rapidly developing group of companies that, among others, has been contributing to the fight against Covid-19.

Since 2016, LabRobot has received every year an award from AddLife thanks to our performance and the confidence entrusted in us from our customers.

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Do you want to appear on our website? We would like to invite our satisfied customers to give us feedback on Dilucup System.

If you are interested, do not hesitate to contact Elisabet asking for more information. Thank you for your kindly collaboration!

Contact: elisabet.nunez@labrobot.com