The most important thing when performing serial dilutions is that the volume is accurate. Otherwise, you can forget about having a good result because the accumulated error will give you a wrong enumeration of microorganisms.

Volume statistics (based on 25.000 measurements):

• 94,4 % -> 9 mL ± 0.5 %
• 99,95 % -> 9 mL ± 1 %
• 100 % -> 9 mL ± 2 % (according to ISO standards)

Methods where filling tubes or other recipients is done in the laboratory cannot guarantee a volume as accurate as Dilucup.